his work presents a framework that combines OCT imaging and DVC to obtain accurate measurements of full three-dimensional displacement fields inside skeletal muscle at the microscopic scale. Using high-resolution OCT volumes, the method captures real-time internal deformation of the EDL muscle under load. DVC analysis provides precise, non-invasive quantification of fiber-level kinematics. The technique reveals internal mechanical interactions that are inaccessible with conventional methods. This combined framework offers a major advance toward non-destructive, experimentally grounded muscle characterization and establishes the foundational first step toward a high-fidelity, geometry multiscale numerical model of skeletal muscle mechanical behavior.